Ultrasound technology is probably offered at numerous institutions that are zoological universities where frogs could be housed, however it could be extremely hard to visualize the specific reproductive organs (testes and ovaries) of anurans 24, 26. While this research discovered that ultrasound imaging had been fairly effective in properly determining the intercourse of DGFs whenever performed by an observer with extensive amphibian imaging experience, it may be argued that this system could be of restricted used to those scientists with just minimal amphibian experience that is ultrasound. The developing hair follicles of females will be the many noticeable and simple to differentiate on ultrasound images; consequently, when it comes to typical observer, the accuracy that is greatest in making use of ultrasound imaging could be limited by females with well-developed hair follicles. For many anuran species, specially the ones that are very regular inside their reproduction, effective imaging for the hair follicles could be restricted to certain periods, since the follicles just grow and develop during specific times during the the entire year 7. Seasonality would not appear be a concern for DGFs in this research, as females had been seen to own development that is follicular the entire year centered on regular ultrasound imaging (Graham, unpublished information); nonetheless, these females had been additionally periodically treated with exogenous hormones for separate studies therefore it is unknown exactly exactly how this might have impacted their normal pattern of follicular growth and development. With this research, just the single image that is ultrasound the start of the research (just before any hormones management) ended up being utilized to prevent alterations in the ultrasound pictures of this females from the hormonally induced development and deposition of eggs.